Fluorescence lifetime spectroscopy and imaging (UEDIN)
Light microscopy
Description
The fluorescence lifetime facility is based around a femto-second laser system which can produce excitation light from the UV to the blue visible range (as well as near infrared light). A pulse picker can reduce the repetition rate from ~5MHz to less than 1 kHz, making it also suitable for dyes with long lifetimes.
This excitation source is predominantly used for fluorescence lifetime spectroscopy to measure spectrally resolved fluorescent lifetimes with about 100ps resolution using a multi-channel plate for time correlated single photon counting (TCSPC). The Edinburgh Instruments fluorescence lifetime spectrometer has a standard sample chamber with cuvette holders as well as a second chamber which allows the use of custom excitation geometries.
Alternatively the laser system can be used with two different time domain fluorescence lifetime imaging systems. Both are wide
field systems based on either a gated CCD camera or a much more quantitative spatially resolved TCSPC system.
Equipment details
Laser system
- Coherent Verdi V10 pump laser
- Coherent Mira 900 Ti:Sapphire laser (700-1000 nm, 76 MHz)
- Pulse Picker
- Harmonic Generator (2nd & 3rd harmonic)
Edinburgh Instrument Spectrometer
- 2 sample chambers
- chamber with standard cuvette holders
- chamber for custom excitation geometries
- 2 detectors
- Hamamatsu microchannel plate photomultiplier (R3809U-50)
- Hamamatsu photosensor module (H7422)
- TCC900 photon counting electronics
- F900 instrument control software
Fluorescence lifetime imaging
- Nikon Eclipse TE300 inverted microscope
- LaVision PicoStar Multi-Channel Plate (MCP) Intenfied CCD Camera
- Europhoton Quandrant-Anode Photomultiplier Tube (QA-PMT)
Local Contacts
