Fluorescence lifetime spectroscopy and imaging (UEDIN)

Light microscopy

Description

The fluorescence lifetime facility is based around a femto-second laser system which can produce excitation light from the UV to the blue visible range (as well as near infrared light). A pulse picker can reduce the repetition rate from ~5MHz to less than 1 kHz, making it also suitable for dyes with long lifetimes. 

This excitation source is predominantly used for fluorescence lifetime spectroscopy to measure spectrally resolved fluorescent lifetimes with about 100ps resolution using a multi-channel plate for time correlated single photon counting (TCSPC). The Edinburgh Instruments fluorescence lifetime spectrometer has a standard sample chamber with cuvette holders as well as a second chamber which allows the use of custom excitation geometries.


Alternatively the laser system can be used with two different time domain fluorescence lifetime imaging systems. Both are wide
field systems based on either a gated CCD camera or a much more quantitative spatially resolved TCSPC system.

 

Equipment details

Laser system

Edinburgh Instrument Spectrometer

 

Fluorescence lifetime imaging

 

 

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