800 MHz spectrometer (1H resonance frequency 800 MHz) (AMU)
NMR
Description
With the Agilent 800 MHz spectrometer, structural properties of peptides, proteins, RNA or DNA in solution can be studied. Multidimensional (up to 5D) NMR experiments on 15N-, 13C,15N-, and 2H13C15N-triple labeled proteins can be conducted with molecular masses up to 40 kDa. The research dealing with recording, processing and analysis of high-dimensionality (4D, 5D) NMR data sets acquired with nonlinear sampling in evolution time-space can be carried out. The group staff has great experience in collection and studies of 15N relaxation (R1, R2 and 1H-15N NOE) data sets with several magnetic fields.
The 18.8 T, narrow bore 54 mm superconductive magnet provides a strong, stable and homogeneous external magnetic field. The system is equipped with four different NMR probes:
- 1H{13C/15N}, 5mm, PFG, Triple Resonance Probe designed for triple resonance experiments of biomolecular samples (peptides, nucleic acids). It enables simultaneous irradiation of carbon 13C and nitrogen 15N. It is equipped with automatic tuning unit. Variable temperature range -20 °C ÷ 80 °C.
- X{1H}, 5mm, PFG, Broadband Probe for double resonance studies. It enables observation of low frequency broadband nuclei (X → 15N, 13C, 31P). Variable temperature range -80 °C ÷ 120 °C.
- Wide Bore Triple Resonance T3 MAS XY{1H-19F}, 1.6 mm, designed for solid state measurements. It has a triply tuned circuit for three channel operation. Both the high frequency and the low frequency channels can be used for detection or decoupling. The low frequency channels can be tuned for various nuclei (X →31P - 79Br, Y → 23Na – 15N). An integrated rotor module (MAS) which operates with 1.6 mm rotor diameter allows one to spin the sample up to 40 kHz. Variable temperature range -40 °C ÷ 60 °C.
- Wide Bore Triple Resonance T3 MAS XY{1H-19F}, 3.2 mm, designed for solid state measurements. It has a triply tuned circuit for three channel operation. Both the high frequency and the low frequency channels can be used for detection or decoupling. The low frequency channels can be tuned for various nuclei (X →31P - 79Br, Y → 23Na – 15N). An integrated rotor module (MAS) which operates with 3.2 mm rotor diameter allows one to spin the sample up to 25 kHz. Variable temperature range -75 °C ÷ 100 °C.
Local Contacts
- Prof. Stefan Jurga
- Prof. Kosma Szutkowski
